Hotspot

trnT-tRNA-Leu

LSC · 46271-47033 · 763 bp

Back to Pleuropogon

Pi 0.026

MarkerSeek score 67.60

Primer available yes

Label trnT-tRNA-Leu

Feature Metadata

label_name: trnT-tRNA-Leu
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 36
pi: 0.026
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-tRNA-Leu_p1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.0	58.0	862-881 bp	1.0	83.6
trnT-tRNA-Leu_p2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	50.0	50.0	58.1	58.0	854-873 bp	1.0	83.3
trnT-tRNA-Leu_p3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	55.0	50.0	57.9	58.0	832-851 bp	1.0	82.9
trnT-tRNA-Leu_p4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	58.0	58.2	852-871 bp	1.0	81.7
trnT-tRNA-Leu_p5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	50.0	50.0	58.0	57.7	861-880 bp	1.0	81.4