Hotspot

trnfM-tRNA-Gly

LSC · 12536-12630 · 95 bp

Back to Pleuropogon

Pi 0.0421

MarkerSeek score 66.50

Primer available yes

Label trnfM-tRNA-Gly

Feature Metadata

label_name: trnfM-tRNA-Gly
feature_type: igs
region: LSC
length_bp: 95
variable_sites: 7
pi: 0.0421
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-tRNA-Gly_p1	CCTCAAGGTTATGAGCCTCG	AGCCTTCCAAGCTAACGATG	55.0	50.0	57.8	58.0	181-186 bp	1.0	82.7
trnfM-tRNA-Gly_p2	CCTCGTGAGCTACCAAACTG	AGCCTTCCAAGCTAACGATG	55.0	50.0	58.3	58.0	166-171 bp	1.0	82.7
trnfM-tRNA-Gly_p3	CCTCAAGGTTATGAGCCTCG	CCTAGCCTTCCAAGCTAACG	55.0	55.0	57.8	58.1	184-189 bp	1.0	82.4
trnfM-tRNA-Gly_p4	CCTCGTGAGCTACCAAACTG	CCTAGCCTTCCAAGCTAACG	55.0	55.0	58.3	58.1	169-174 bp	1.0	82.4
trnfM-tRNA-Gly_p5	CCTCAAGGTTATGAGCCTCG	CCAACGTCGACTATAACCCC	55.0	55.0	57.8	57.8	202-207 bp	1.0	80.7