Hotspot

trnG-trnfM

LSC · 11983-12462 · 480 bp

Back to Pleuropogon

Pi 0.0297

MarkerSeek score 71.20

Primer available yes

Label trnG-trnfM

Feature Metadata

label_name: trnG-trnfM
feature_type: igs
region: LSC
length_bp: 480
variable_sites: 24
pi: 0.0297
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnfM_p1	AGGAGAAGATACGGGTTCGA	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.6	57.8	547-581 bp	1.0	81.1
trnG-trnfM_p2	AGGAGAAGATACGGGTTCGA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	57.6	58.3	562-596 bp	1.0	80.5
trnG-trnfM_p3	AGGAGAAGATACGGGTTCGA	GAGCGGAGTAGAGCAGTTTG	50.0	55.0	57.6	58.4	575-609 bp	1.0	79.7
trnG-trnfM_p4	AGGAGAAGATACGGGTTCGA	AGAGCGGAGTAGAGCAGTTT	50.0	50.0	57.6	58.4	576-610 bp	1.0	79.1
trnG-trnfM_p5	TTGCCAAGGAGAAGATACGG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.3	57.8	553-587 bp	1.0	79.0