Hotspot

trnS-psbD

LSC · 7554-8441 · 888 bp

Back to Pleuropogon

Pi 0.0216

MarkerSeek score 64.80

Primer available yes

Label trnS-psbD

Feature Metadata

label_name: trnS-psbD
feature_type: igs
region: LSC
length_bp: 888
variable_sites: 32
pi: 0.0216
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psbD_p1	ATTAGCAATCCGCCGCTTTA	AGGAATTCTACCAAGGGCTA	45.0	45.0	58.0	55.2	955-1042 bp	1.0	62.9
trnS-psbD_p2	ATTAGCAATCCGCCGCTTTA	TAGGAATTCTACCAAGGGCT	45.0	45.0	58.0	55.2	956-1043 bp	1.0	62.9
trnS-psbD_p3	ATTAGCAATCCGCCGCTTTA	TTAGGAATTCTACCAAGGGCT	45.0	42.9	58.0	56.0	957-1044 bp	1.0	61.6
trnS-psbD_p4	GAGCGGAAAGAGAGGGATTC	AGGAATTCTACCAAGGGCTA	55.0	45.0	57.8	55.2	1006-1093 bp	1.0	61.1
trnS-psbD_p5	GAGCGGAAAGAGAGGGATTC	TAGGAATTCTACCAAGGGCT	55.0	45.0	57.8	55.2	1007-1094 bp	1.0	61.1