Hotspot

trnQ-psbI

LSC · 6328-7234 · 907 bp

Back to Pleuropogon

Pi 0.0186

MarkerSeek score 64.50

Primer available yes

Label trnQ-psbI

Feature Metadata

label_name: trnQ-psbI
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 27
pi: 0.0186
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-psbI_p1	GATCAAAACCCGCTGCCTTA	ACGTCCTGGGTCATTGGATA	50.0	50.0	58.5	58.1	1035-1058 bp	1.0	76.7
trnQ-psbI_p2	GATCAAAACCCGCTGCCTTA	TACGTCCTGGGTCATTGGAT	50.0	50.0	58.5	58.1	1036-1059 bp	1.0	76.7
trnQ-psbI_p3	GATCAAAACCCGCTGCCTTA	TTTTTACTCCTCACGCCCAG	50.0	50.0	58.5	57.8	1059-1082 bp	1.0	76.3
trnQ-psbI_p4	GATCAAAACCCGCTGCCTTA	TTACGTCCTGGGTCATTGGA	50.0	50.0	58.5	58.4	1037-1060 bp	1.0	74.9
trnQ-psbI_p5	ATCAAAACCCGCTGCCTTAC	ACGTCCTGGGTCATTGGATA	50.0	50.0	58.8	58.1	1034-1057 bp	1.0	74.4