Hotspot

rpoC2

LSC · 18125-22294 · 4170 bp

Back to Pleurophora

Pi 0.00416

MarkerSeek score 44.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4170
variable_sites: 26
pi: 0.00416
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TCCTCGAAATGATTGTTCTGGT	GGTCGGATCGATTGGTTGAA	40.9	50.0	57.7	58.0	243 bp	1.0	62.9
rpoC2_p2	CCTCGAAATGATTGTTCTGGTG	GGTCGGATCGATTGGTTGAA	45.5	50.0	57.7	58.0	242 bp	1.0	62.4
rpoC2_p3	CCTCGAAATGATTGTTCTGGT	GGTCGGATCGATTGGTTGAA	42.9	50.0	56.2	58.0	242 bp	1.0	59.1
rpoC2_p4	TCCTCGAAATGATTGTTCTGGT	ATCGATTGGTTGAAAGGCCT	40.9	45.0	57.7	57.5	237 bp	1.0	58.9
rpoC2_p5	CCTCGAAATGATTGTTCTGGTG	ATCGATTGGTTGAAAGGCCT	45.5	45.0	57.7	57.5	236 bp	1.0	58.5