Hotspot

ndhG-ndhI

SSC · 124516-124953 · 438 bp

Back to Pleurophora

Pi 0.0107

MarkerSeek score 62.60

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 438
variable_sites: 7
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATTGGTCCAGGCAAATCCAT	TCAAATTGCTTTGGGTCGTT	45.0	40.0	57.4	56.4	541-543 bp	1.0	64.4
ndhG-ndhI_p2	ATTGGTCCAGGCAAATCCAT	TCGACTTATGATCGTCACGA	45.0	45.0	57.4	56.2	573-575 bp	1.0	62.7
ndhG-ndhI_p3	ATTGGTCCAGGCAAATCCAT	ATCAAATTGCTTTGGGTCGT	45.0	40.0	57.4	56.2	542-544 bp	1.0	62.6
ndhG-ndhI_p4	ATTGGTCCAGGCAAATCCAT	TCAAATTGCTTTGGGTCGTTT	45.0	38.1	57.4	57.2	541-543 bp	1.0	62.4
ndhG-ndhI_p5	ATTGGTCCAGGCAAATCCAT	TCGACTTATGATCGTCACGAA	45.0	42.9	57.4	57.0	573-575 bp	1.0	60.6