Hotspot

trnL-UAG-ccsA

SSC · 119824-119930 · 107 bp

Back to Pleurophora

Pi 0.0187

MarkerSeek score 63.90

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 107
variable_sites: 3
pi: 0.0187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	GTCCTCTGATTTCATCGACTGA	50.0	45.5	58.0	57.6	283-287 bp	1.0	62.9
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	GTCCTCTGATTTCATCGACTGA	50.0	45.5	58.0	57.6	282-286 bp	1.0	62.9
trnL-UAG-ccsA_p3	GTGAAATCGGTAGACACGCT	AGTCCTCTGATTTCATCGACTG	50.0	45.5	58.0	57.6	284-288 bp	1.0	62.8
trnL-UAG-ccsA_p4	TGAAATCGGTAGACACGCTG	AGTCCTCTGATTTCATCGACTG	50.0	45.5	58.0	57.6	283-287 bp	1.0	62.8
trnL-UAG-ccsA_p5	TAGACACGCTGCTCTTAGGA	GTCCTCTGATTTCATCGACTGA	50.0	45.5	57.9	57.6	273-277 bp	1.0	62.0