Hotspot

psbT-pbf1

LSC · 79899-79957 · 59 bp

Back to Pleurophora

Pi 0.0678

MarkerSeek score 87.50

Primer available yes

Label psbT-pbf1

Feature Metadata

label_name: psbT-pbf1
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 6
pi: 0.0678
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-pbf1_p1	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	47.6	55.0	57.6	58.4	125 bp	1.0	69.2
psbT-pbf1_p2	CGAGAACCACCTAAAGTTCCA	AGATCCATTCGAGGAACACG	47.6	50.0	57.6	57.4	126 bp	1.0	67.9
psbT-pbf1_p3	CGAGAACCACCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	47.6	50.0	57.6	57.4	148 bp	1.0	67.6
psbT-pbf1_p4	TCGAGAACCACCTAAAGTTCC	GATCCATTCGAGGAACACGG	47.6	55.0	57.3	58.4	126 bp	1.0	67.0
psbT-pbf1_p5	TCGAGAACCACCTAAAGTTCC	GGCAACCCTCTCAACAACTA	47.6	50.0	57.3	57.4	149 bp	1.0	65.3