Hotspot

rpl33-rps18

LSC · 73285-73631 · 347 bp

Back to Pleurophora

Pi 0.0215

MarkerSeek score 59.00

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 347
variable_sites: 10
pi: 0.0215
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGATTCACGGGGAGATAAAGA	AGAATCGCTTGGATTTATCC	47.6	40.0	57.3	53.1	392-400 bp	1.0	45.9
rpl33-rps18_p2	CGATTCACGGGGAGATAAAGA	AGAAAGAATCGCTTGGATTTATCC	47.6	37.5	57.3	57.4	396-404 bp	1.0	45.8
rpl33-rps18_p3	CGATTCACGGGGAGATAAAGA	AGAAAGAATCGCTTGGATTT	47.6	35.0	57.3	53.2	396-404 bp	1.0	45.8
rpl33-rps18_p4	CGATTCACGGGGAGATAAAGAA	AGAAAGAATCGCTTGGATTTATCC	45.5	37.5	57.9	57.4	396-404 bp	1.0	45.8
rpl33-rps18_p5	TCGGCATAATACTCCTAGTCGA	AGAAAGAATCGCTTGGATTTATCC	45.5	37.5	58.0	57.4	458-466 bp	1.0	45.0