Hotspot

psaJ-rpl33

LSC · 72518-73083 · 566 bp

Back to Pleurophora

Pi 0.00827

MarkerSeek score 65.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 7
pi: 0.00827
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATCGATTATTCCCGGATGCG	GGACTTCTTTACCCTTGGCC	50.0	55.0	58.0	58.2	633-639 bp	1.0	80.7
psaJ-rpl33_p2	ACATATCTTTCCGTGGCACC	GGACTTCTTTACCCTTGGCC	50.0	55.0	58.0	58.2	706-712 bp	1.0	80.3
psaJ-rpl33_p3	TACTCTATGGTTCGGGGCTT	GGACTTCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	677-683 bp	1.0	79.3
psaJ-rpl33_p4	ATCGATTATTCCCGGATGCG	TTACGCGGACTTCTTTACCC	50.0	50.0	58.0	57.6	639-645 bp	1.0	79.1
psaJ-rpl33_p5	TGGCACCGGTACTAAGTACT	GGACTTCTTTACCCTTGGCC	50.0	55.0	57.8	58.2	693-699 bp	1.0	78.9