Hotspot

psaA-pafI

LSC · 45207-45909 · 703 bp

Back to Pleurophora

Pi 0.00948

MarkerSeek score 65.10

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 10
pi: 0.00948
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	900-904 bp	1.0	80.0
psaA-pafI_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	881-885 bp	1.0	79.8
psaA-pafI_p3	TGAGAAATGACCCGGTTTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	902-906 bp	1.0	79.2
psaA-pafI_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	883-887 bp	1.0	79.0
psaA-pafI_p5	TGAGAAATGACCCGGTTTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	896-900 bp	1.0	78.2