Hotspot

trnG-GCC-trnfM-CAU

LSC · 39863-40062 · 200 bp

Back to Pleurophora

Pi 0.03

MarkerSeek score 66.00

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 200
variable_sites: 9
pi: 0.03
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	278 bp	1.0	80.9
trnG-GCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	CTCCTTCTTAGCGGGGTAGA	50.0	55.0	57.8	57.9	327 bp	1.0	80.3
trnG-GCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	TCCTTCTTAGCGGGGTAGAG	50.0	55.0	57.8	57.9	326 bp	1.0	80.3
trnG-GCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	276 bp	1.0	79.8
trnG-GCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	298 bp	1.0	79.3