Hotspot

rps2-rpoC2

LSC · 17900-18124 · 225 bp

Back to Pleurophora

Pi 0.0341

MarkerSeek score 69.40

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 225
variable_sites: 11
pi: 0.0341
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	AGGTGCCATCCTAGGATTCC	CGCGGGCGAAATCATAGATA	55.0	50.0	58.6	58.0	437-446 bp	1.0	77.8
rps2-rpoC2_p2	TGCCGAAATATAAGGTGCCA	CGCGGGCGAAATCATAGATA	45.0	50.0	57.3	58.0	449-458 bp	1.0	76.5
rps2-rpoC2_p3	GGTGCCATCCTAGGATTCCA	CGCGGGCGAAATCATAGATA	55.0	50.0	58.9	58.0	436-445 bp	1.0	75.4
rps2-rpoC2_p4	TTGCCGAAATATAAGGTGCCA	CGCGGGCGAAATCATAGATA	42.9	50.0	58.0	58.0	450-459 bp	1.0	74.2
rps2-rpoC2_p5	TGCCGAAATATAAGGTGCCAT	CGCGGGCGAAATCATAGATA	42.9	50.0	57.8	58.0	449-458 bp	1.0	72.4