Hotspot

trnR-UCU-atpA

LSC · 11162-11520 · 359 bp

Back to Pleurophora

Pi 0.0288

MarkerSeek score 74.00

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 359
variable_sites: 15
pi: 0.0288
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CAGGAACAGAAGGAACGCTT	47.6	50.0	57.3	58.1	420-427 bp	1.0	70.1
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCAGGAACAGAAGGAACGC	47.6	50.0	57.3	58.1	422-429 bp	1.0	70.0
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	CATTTACCCCGGAAGCAGAA	47.6	50.0	57.3	57.9	461-468 bp	1.0	69.6
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AACATTTACCCCGGAAGCAG	47.6	50.0	57.3	58.2	463-470 bp	1.0	69.2
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	AAACATTTACCCCGGAAGCA	47.6	45.0	57.3	57.7	464-471 bp	1.0	68.2