Hotspot

psbK-psbI

LSC · 8571-8990 · 420 bp

Back to Pleurophora

Pi 0.0103

MarkerSeek score 62.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 420
variable_sites: 6
pi: 0.0103
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	554-558 bp	1.0	79.0
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	551-555 bp	1.0	77.0
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	552-556 bp	1.0	77.0
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	553-557 bp	1.0	76.9
psbK-psbI_p5	TGTTTGGCAAGCTGCTGTAA	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.6	57.6	555-559 bp	1.0	74.6