Hotspot

psbC

LSC · 34317-35701 · 1385 bp

Back to Pleroma

Pi 0.00581

MarkerSeek score 62.70

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1385
variable_sites: 8
pi: 0.00581
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCCCTGAGGAGGTTCTACC	TTTGAAGTCTGGCCTTGACC	55.0	50.0	57.8	58.0	1499-1500 bp	1.0	78.8
psbC_p2	AGGTATTCGTGCATGGATGG	TTTGAAGTCTGGCCTTGACC	50.0	50.0	57.7	58.0	1551-1552 bp	1.0	78.6
psbC_p3	TTCCCTGAGGAGGTTCTACC	GCCAGGGGAATCAAATGGAT	55.0	50.0	57.8	57.9	1456-1457 bp	1.0	78.2
psbC_p4	AGGTATTCGTGCATGGATGG	GCCAGGGGAATCAAATGGAT	50.0	50.0	57.7	57.9	1508-1509 bp	1.0	78.1
psbC_p5	TTCCCTGAGGAGGTTCTACC	ATGCCAGGGGAATCAAATGG	55.0	50.0	57.8	58.2	1458-1459 bp	1.0	77.3