Hotspot

rps15-trnN-GUU

SSC · 124822-131141 · 6320 bp

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Pi 0.00237
MarkerSeek score 53.90
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
6320
variable_sites
15
pi
0.00237
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 AGTTTGTGAAATCCAACGAAGA CCTTTTGGTTCTCCCCGAAA 36.4 50.0 56.5 58.0 263 bp 1.0 53.1
rps15-trnN-GUU_p2 AGTTTGTGAAATCCAACGAAGA CCTTCCTTTTGGTTCTCCCC 36.4 55.0 56.5 58.1 267 bp 1.0 52.5
rps15-trnN-GUU_p3 AGTTTGTGAAATCCAACGAAGA TTTTGGTTCTCCCCGAAACC 36.4 50.0 56.5 58.3 261 bp 1.0 50.7
rps15-trnN-GUU_p4 AGTTTGTGAAATCCAACGAAGAA CCTTTTGGTTCTCCCCGAAA 34.8 50.0 57.2 58.0 263 bp 1.0 50.5
rps15-trnN-GUU_p5 AGTTTGTGAAATCCAACGAAGAA CCTTCCTTTTGGTTCTCCCC 34.8 55.0 57.2 58.1 267 bp 1.0 50.0