Hotspot

ndhG-ndhI

SSC · 120047-120525 · 479 bp

Back to Pleroma

Pi 0.0227

MarkerSeek score 60.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 479
variable_sites: 10
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ATTGGTCCAGGCAAATCCAT	TCAAATCGCATTAGGTCGGT	45.0	45.0	57.4	57.3	557-596 bp	1.0	75.5
ndhG-ndhI_p2	ATTGGTCCAGGCAAATCCAT	TCAAATCGCATTAGGTCGGTT	45.0	42.9	57.4	58.0	557-596 bp	1.0	72.8
ndhG-ndhI_p3	ATTGGTCCAGGCAAATCCAT	ATCAAATCGCATTAGGTCGGT	45.0	42.9	57.4	57.8	558-597 bp	1.0	71.4
ndhG-ndhI_p4	TCATGTATTGGTCCAGGCAA	TCAAATCGCATTAGGTCGGT	45.0	45.0	56.8	57.3	563-602 bp	1.0	70.4
ndhG-ndhI_p5	TATTGGTCCAGGCAAATCCA	TCAAATCGCATTAGGTCGGT	45.0	45.0	56.8	57.3	558-597 bp	1.0	70.2