Hotspot

ndhF-rpl32

SSC · 113938-114905 · 968 bp

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Pi 0.00633

MarkerSeek score 64.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 968
variable_sites: 6
pi: 0.00633
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGGGAAGTGGAATGAGAGGT	TGAAAAAGCCTTCAACGCTG	50.0	45.0	58.0	57.5	1102-1119 bp	1.0	77.6
ndhF-rpl32_p2	TGGGAAGTGGAATGAGAGGT	GAAAAAGCCTTCAACGCTGT	50.0	45.0	58.0	57.5	1101-1118 bp	1.0	77.6
ndhF-rpl32_p3	TGGGAAGTGGAATGAGAGGT	ATGAAAAAGCCTTCAACGCTG	50.0	42.9	58.0	58.0	1103-1120 bp	1.0	73.4
ndhF-rpl32_p4	TGGGAAGTGGAATGAGAGGT	TCAACGCTGTAAAATAGGCCT	50.0	42.9	58.0	57.7	1091-1108 bp	1.0	70.8
ndhF-rpl32_p5	CCACATGCGTCGAAGATTTT	TGAAAAAGCCTTCAACGCTG	45.0	45.0	57.1	57.5	1170-1187 bp	1.0	70.6