Hotspot

psbE-petL

LSC · 64929-66090 · 1162 bp

Back to Pleroma

Pi 0.000907

MarkerSeek score 64.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1162
variable_sites: 1
pi: 0.000907
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	TGACAAATAACCAACCCGCA	GGGTTCGGCTCAGTCAATTA	45.0	50.0	58.0	57.6	1342-1396 bp	1.0	80.3
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	GGGTTCGGCTCAGTCAATTA	50.0	50.0	57.8	57.6	1254-1308 bp	1.0	79.4
psbE-petL_p3	GTTCTCCTGTGCTTCCAGAC	GGGTTCGGCTCAGTCAATTA	55.0	50.0	58.2	57.6	1249-1303 bp	1.0	79.1
psbE-petL_p4	TCTCCTGTGCTTCCAGACAT	GGGTTCGGCTCAGTCAATTA	50.0	50.0	58.4	57.6	1247-1301 bp	1.0	77.9
psbE-petL_p5	TTCTCCTGTGCTTCCAGACA	GGGTTCGGCTCAGTCAATTA	50.0	50.0	58.6	57.6	1248-1302 bp	1.0	76.0