Hotspot

petA-psbJ

LSC · 63684-64121 · 438 bp

Back to Pleroma

Pi 0.0114

MarkerSeek score 61.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 438
variable_sites: 5
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCCGTGTCCAAGGTCTTTTG	ATACTCAGGGTTGGGCTCAT	50.0	50.0	58.0	58.1	579 bp	1.0	79.6
petA-psbJ_p2	CATTCCGTGTCCAAGGTCTT	ATACTCAGGGTTGGGCTCAT	50.0	50.0	57.8	58.1	582 bp	1.0	78.4
petA-psbJ_p3	TCCGTGTCCAAGGTCTTTTG	GTTCATACTCAGGGTTGGGC	50.0	55.0	58.0	58.2	583 bp	1.0	78.4
petA-psbJ_p4	TCCGTGTCCAAGGTCTTTTG	TTCATACTCAGGGTTGGGCT	50.0	50.0	58.0	58.3	582 bp	1.0	77.7
petA-psbJ_p5	CCGTGTCCAAGGTCTTTTGT	ATACTCAGGGTTGGGCTCAT	50.0	50.0	58.3	58.1	578 bp	1.0	77.3