Hotspot

ndhC-trnC-ACA

LSC · 51513-52290 · 778 bp

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Pi 0.0013
MarkerSeek score 60.50
Primer available yes
Label ndhC-trnC-ACA

Feature Metadata

label_name
ndhC-trnC-ACA
feature_type
igs
region
LSC
length_bp
778
variable_sites
1
pi
0.0013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnC-ACA_p1 GGACCTTTACTAATCGGGGC GGTTCGAATCCGTATAGCCC 55.0 55.0 57.7 57.9 906-913 bp 1.0 78.2
ndhC-trnC-ACA_p2 AATCGGGGCTAAAATTCCGG GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 895-902 bp 1.0 77.7
ndhC-trnC-ACA_p3 GGACCTTTACTAATCGGGGC CGGTTCGAATCCGTATAGCC 55.0 55.0 57.7 58.3 907-914 bp 1.0 76.3
ndhC-trnC-ACA_p4 CGGGGCTAAAATTCCGGAAA GGTTCGAATCCGTATAGCCC 50.0 55.0 58.5 57.9 892-899 bp 1.0 75.9
ndhC-trnC-ACA_p5 AATCGGGGCTAAAATTCCGG CGGTTCGAATCCGTATAGCC 50.0 55.0 58.3 58.3 896-903 bp 1.0 75.9