Hotspot

psaA-ycf3

LSC · 42537-43144 · 608 bp

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Pi 0.00493

MarkerSeek score 61.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 608
variable_sites: 3
pi: 0.00493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	786 bp	1.0	79.1
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	GGAAACAGGCTATAGCGCTT	50.0	50.0	57.9	58.0	768 bp	1.0	79.0
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TCACAAGGCGCTTCGAATAA	50.0	45.0	57.9	57.9	708 bp	1.0	78.7
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACAAGGCGCTT	50.0	45.0	57.9	58.1	715 bp	1.0	78.6
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGCCGCTGAGTATTGGAAAC	50.0	50.0	57.9	58.3	782 bp	1.0	77.3