Hotspot

trnT-GGU-psbD

LSC · 31977-33271 · 1295 bp

Back to Pleroma

Pi 0.000804

MarkerSeek score 61.10

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1295
variable_sites: 1
pi: 0.000804
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	AGGAGCCCCTTTAACTCAGT	GAAACGGTCCCTACGTAACC	50.0	55.0	58.0	58.0	1404-1455 bp	1.0	82.5
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1386-1437 bp	1.0	82.4
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1389-1440 bp	1.0	82.2
trnT-GGU-psbD_p4	GAGCCCCTTTAACTCAGTGG	GAAACGGTCCCTACGTAACC	55.0	55.0	57.9	58.0	1402-1453 bp	1.0	82.0
trnT-GGU-psbD_p5	GGAGCCCCTTTAACTCAGTG	GAAACGGTCCCTACGTAACC	55.0	55.0	57.9	58.0	1403-1454 bp	1.0	82.0