Hotspot

petN-psbM

LSC · 28924-29298 · 375 bp

Back to Pleroma

Pi 0.00283

MarkerSeek score 60.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 1
pi: 0.00283
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	45.0	42.9	58.0	57.0	508-530 bp	1.0	67.7
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.8	57.0	514-536 bp	1.0	66.2
petN-psbM_p3	TCTGTCCCTCGTAGTATGGG	TGCTACTGCACTCTTCATTCT	55.0	42.9	57.6	57.0	469-491 bp	1.0	65.2
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	AAAACCGTGAGTCAAAGTGA	45.0	40.0	58.0	55.4	453-475 bp	1.0	63.5
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	45.0	45.0	58.0	55.5	508-530 bp	1.0	63.4