Hotspot

trnC-GCA-petN

LSC · 28208-28833 · 626 bp

Back to Pleroma

Pi 0.00479

MarkerSeek score 61.50

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 626
variable_sites: 3
pi: 0.00479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	707-942 bp	1.0	77.4
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	CCTTTAGAGTCCACTTCGCC	50.0	55.0	58.4	58.0	766-1001 bp	1.0	77.3
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	CTTTAGAGTCCACTTCGCCC	50.0	55.0	58.4	58.0	765-1000 bp	1.0	77.3
trnC-GCA-petN_p4	TTTTTGTTTGGCGACATGGC	TTAAAGCAGCCCAAGCAAGA	45.0	45.0	58.4	58.0	740-975 bp	1.0	76.8
trnC-GCA-petN_p5	TTTTTGTTTGGCGACATGGC	CTTTAGAGTCCACTTCGCCC	45.0	55.0	58.4	58.0	798-1033 bp	1.0	76.8