Hotspot

ycf1

SSC · 127223-132781 · 5559 bp

Back to Pleione

Pi 0.00942

MarkerSeek score 48.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5559
variable_sites: 228
pi: 0.00942
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGACATTTCATTGCGTAAGGC	TCACTTTAAAGAGGCACGCT	42.9	45.0	57.5	57.5	80-248 bp	1.0	75.4
ycf1_p2	TGACATTTCATTGCGTAAGGC	TCAAGATAGCCCGGTTTACG	42.9	50.0	57.5	57.4	80-227 bp	1.0	75.4
ycf1_p3	TGACATTTCATTGCGTAAGGC	TCTGATCTGGAGACCCATCA	42.9	50.0	57.5	57.1	80-201 bp	1.0	73.3
ycf1_p4	TGACATTTCATTGCGTAAGGC	TCTGGAGACCCATCAAGAGA	42.9	50.0	57.5	57.1	80-196 bp	1.0	72.7
ycf1_p5	TGACATTTCATTGCGTAAGGC	AAAGAGGCACGCTATCAAGA	42.9	45.0	57.5	56.9	80-241 bp	1.0	71.1