Hotspot

rpl32-trnL-UAG

SSC · 116490-117144 · 655 bp

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Pi 0.0137

MarkerSeek score 60.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 655
variable_sites: 35
pi: 0.0137
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	80-785 bp	1.0	86.5
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	80-778 bp	1.0	86.1
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	58.0	57.8	80-784 bp	1.0	86.0
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	CTAAGAGCAGCGTGTCTACC	50.0	55.0	58.0	57.8	80-783 bp	1.0	86.0
rpl32-trnL-UAG_p5	AATCTGTTTCCACCGGACAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.8	57.9	80-793 bp	1.0	85.3