Hotspot

psbB-psbT

LSC · 75717-76240 · 524 bp

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Pi 0.0119

MarkerSeek score 52.90

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 524
variable_sites: 33
pi: 0.0119
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	TGGAGATCCAACTACAAGGAGA	TGGAACTTTAGGTGGTTCTCG	45.5	47.6	57.9	57.6	80-648 bp	1.0	61.3
psbB-psbT_p2	TGGAGATCCAACTACAAGGAGA	GGAACTTTAGGTGGTTCTCGA	45.5	47.6	57.9	57.3	80-647 bp	1.0	59.1
psbB-psbT_p3	GGAGATCCAACTACAAGGAGAC	TGGAACTTTAGGTGGTTCTCG	50.0	47.6	57.6	57.6	80-647 bp	1.0	58.6
psbB-psbT_p4	TGGAGATCCAACTACAAGGAG	TGGAACTTTAGGTGGTTCTCG	47.6	47.6	56.5	57.6	80-648 bp	1.0	57.4
psbB-psbT_p5	TGGAGATCCAACTACAAGGAGA	GGAACTTTAGGTGGTTCTCGAA	45.5	45.5	57.9	58.0	80-647 bp	1.0	56.5