Hotspot

clpP-psbB

LSC · 73447-74189 · 743 bp

Back to Pleione

Pi 0.0126

MarkerSeek score 61.30

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 36
pi: 0.0126
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	80-944 bp	1.0	83.0
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	80-939 bp	1.0	82.9
clpP-psbB_p3	CGCACTATACGTCAACCCAA	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	80-862 bp	1.0	82.4
clpP-psbB_p4	TATACGTCAACCCAAGCTGC	CGATACCAAGGCAAACCCAT	50.0	50.0	58.0	58.2	80-857 bp	1.0	82.3
clpP-psbB_p5	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.8	57.9	80-890 bp	1.0	81.9