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psaJ-rpl33

LSC · 68319-68900 · 582 bp

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Pi 0.0122

MarkerSeek score 64.60

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 582
variable_sites: 26
pi: 0.0122
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GATGCCTTGTCATTCCCCTT	AATGCCCGGCGATTCTTTAT	50.0	45.0	57.9	58.0	80-708 bp	1.0	85.8
psaJ-rpl33_p2	GATGCCTTGTCATTCCCCTT	ATGCCCGGCGATTCTTTATT	50.0	45.0	57.9	58.0	80-707 bp	1.0	85.8
psaJ-rpl33_p3	GATGCCTTGTCATTCCCCTT	ACACCCTTTCGAACACAACA	50.0	45.0	57.9	57.9	80-686 bp	1.0	85.1
psaJ-rpl33_p4	TGGTTTGGGTCTTTAGCAGG	AATGCCCGGCGATTCTTTAT	50.0	45.0	57.7	58.0	80-759 bp	1.0	84.5
psaJ-rpl33_p5	TGGTTTGGGTCTTTAGCAGG	ATGCCCGGCGATTCTTTATT	50.0	45.0	57.7	58.0	80-758 bp	1.0	84.5