Hotspot

atpB-rbcL

LSC · 55242-56233 · 992 bp

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Pi 0.0157
MarkerSeek score 57.70
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
LSC
length_bp
992
variable_sites
67
pi
0.0157
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 TAGTGGGATTGCTTCTCAT GGTGACATAAGTCCCTCCCT 42.1 55.0 53.0 58.1 80-1071 bp 1.0 49.5
atpB-rbcL_p2 TAGTGGGATTGCTTCTCAT TGGTGACATAAGTCCCTCCC 42.1 55.0 53.0 58.4 80-1072 bp 1.0 49.5
atpB-rbcL_p3 TAGTGGGATTGCTTCTCAT GTGGTGACATAAGTCCCTCC 42.1 55.0 53.0 57.3 80-1073 bp 1.0 49.5
atpB-rbcL_p4 TAGTGGGATTGCTTCTCAT AAGTCCCTCCCTACAACTCA 42.1 50.0 53.0 57.3 80-1063 bp 1.0 49.5
atpB-rbcL_p5 TAGTGGGATTGCTTCTCAT TCCAACGCTTGCTTTAGTCT 42.1 45.0 53.0 57.5 80-1099 bp 1.0 49.4