Hotspot

rpoB-trnC-GCA

LSC · 27364-28821 · 1458 bp

Back to Pleione

Pi 0.0103

MarkerSeek score 61.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1458
variable_sites: 63
pi: 0.0103
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	80-1634 bp	1.0	81.8
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	CGACACCCAGATTCGAACTG	50.0	55.0	58.2	58.6	80-1658 bp	1.0	80.7
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	TTTTTGATCAGGCGACACCC	50.0	50.0	58.2	58.8	80-1670 bp	1.0	79.8
rpoB-trnC-GCA_p4	AAATTCTTCCGTCAAGCCCT	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.4	57.5	80-1635 bp	1.0	78.5
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	ATAAAGGATTTGCAGTCCCCC	50.0	47.6	58.2	58.0	80-1635 bp	1.0	77.7