Hotspot

trnS-GCU-trnG-GCC

LSC · 8610-9601 · 992 bp

Back to Pleione

Pi 0.0172
MarkerSeek score 69.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
992
variable_sites
71
pi
0.0172
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 80-1133 bp 1.0 61.2
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 80-1132 bp 1.0 61.2
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 80-1136 bp 1.0 60.1
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 80-1135 bp 1.0 60.1
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 80-1134 bp 1.0 60.1