Hotspot

rps16-trnQ-UUG

LSC · 6014-7151 · 1138 bp

Back to Pleione

Pi 0.0126

MarkerSeek score 70.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1138
variable_sites: 67
pi: 0.0126
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	ATCCACAGCCAATCATGTCC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	622-1249 bp	1.0	87.5
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	599-1226 bp	1.0	86.9
rps16-trnQ-UUG_p3	CCACAGCCAATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	620-1247 bp	1.0	86.7
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	610-1237 bp	1.0	86.4
rps16-trnQ-UUG_p5	GGATGTCAGAATCCACAGCC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.3	58.1	632-1259 bp	1.0	85.4