Hotspot

rbcL

LSC · 57004-58437 · 1434 bp

Back to Pleioblastus

Pi 0.00279

MarkerSeek score 36.30

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 9
pi: 0.00279
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGAGTTGTAGGGAGGGACTT	ACTGCATTTCGTAACTGCTT	50.0	40.0	57.3	56.0	1531 bp	1.0	58.5
rbcL_p2	TCATGAGTTGTAGGGAGGGA	ACTGCATTTCGTAACTGCTT	50.0	40.0	57.1	56.0	1534 bp	1.0	56.7
rbcL_p3	TGAGTTGTAGGGAGGGACTT	ACTGCATTTCGTAACTGCTTT	50.0	38.1	57.3	56.7	1531 bp	1.0	56.6
rbcL_p4	CATGAGTTGTAGGGAGGGAC	ACTGCATTTCGTAACTGCTT	55.0	40.0	57.0	56.0	1533 bp	1.0	56.3
rbcL_p5	TCATGAGTTGTAGGGAGGGA	ACTGCATTTCGTAACTGCTTT	50.0	38.1	57.1	56.7	1534 bp	1.0	54.8