Hotspot

clpP

LSC · 70274-70924 · 651 bp

Back to Pleioblastus

Pi 0.00529

MarkerSeek score 48.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 651
variable_sites: 8
pi: 0.00529
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTTACAAGAAATCCGGCCA	ACTGCGGATTCTTTCTTTCTCT	40.0	40.9	55.3	57.7	758 bp	1.0	41.1
clpP_p2	AATTTACAAGAAATCCGGCCA	ACTGCGGATTCTTTCTTTCTCT	38.1	40.9	56.1	57.7	759 bp	1.0	40.8
clpP_p3	ATTTACAAGAAATCCGGCCA	GCGGATTCTTTCTTTCTCTTCC	40.0	45.5	55.3	57.4	755 bp	1.0	40.8
clpP_p4	TCGCAGAAAACCTGAATTTAGG	ACTGCGGATTCTTTCTTTCTCT	40.9	40.9	57.0	57.7	790 bp	1.0	40.8
clpP_p5	AAATTTACAAGAAATCCGGCCA	ACTGCGGATTCTTTCTTTCTCT	36.4	40.9	56.9	57.7	760 bp	1.0	40.8