Hotspot

rpl32-trnL-UAG

SSC · 108482-109209 · 728 bp

Back to Pleioblastus

Pi 0.00366
MarkerSeek score 52.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
728
variable_sites
6
pi
0.00366
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 775-800 bp 1.0 81.2
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 774-799 bp 1.0 81.2
rpl32-trnL-UAG_p3 AAGATCATTTTCCAGCGGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 812-837 bp 1.0 80.7
rpl32-trnL-UAG_p4 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 768-793 bp 1.0 80.7
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 767-792 bp 1.0 80.7