Hotspot

psbB-psbT

LSC · 72946-73115 · 170 bp

Back to Pleioblastus

Pi 0

MarkerSeek score 19.60

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 19.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGGGATGGAATAGCTGTTGG	TCGCCATCTCCATATCTGGT	55.0	50.0	58.0	58.0	970 bp	1.0	80.3
psbB-psbT_p2	GGGGATGGAATAGCTGTTGG	AGGTGGTTCTCGGAAGAAGA	55.0	50.0	58.0	58.0	782 bp	1.0	79.9
psbB-psbT_p3	CGGGCTACTTTGAAATCGGA	AGGTGGTTCTCGGAAGAAGA	50.0	50.0	58.0	58.0	479 bp	1.0	79.9
psbB-psbT_p4	TCGGGCTACTTTGAAATCGG	AGGTGGTTCTCGGAAGAAGA	50.0	50.0	58.0	58.0	480 bp	1.0	79.9
psbB-psbT_p5	GGGGATGGAATAGCTGTTGG	ACCTTAGGTGGTTCTCGGAA	55.0	50.0	58.0	58.0	787 bp	1.0	79.8