Hotspot

ycf1

SSC · 127253-132835 · 5583 bp

Back to Plectranthus

Pi 0.0262

MarkerSeek score 53.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5583
variable_sites: 146
pi: 0.0262
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCCGAAAGTCTGATTGTTGC	ATGGAATCGACCATTTCGCT	50.0	45.0	58.0	57.7	1019 bp	1.0	80.6
ycf1_p2	AACCTTTGATTAGACCCCGC	ATGGAATCGACCATTTCGCT	50.0	45.0	57.9	57.7	1037 bp	1.0	79.9
ycf1_p3	AATCCTCAGTCCATTCGGGA	ATGGAATCGACCATTTCGCT	50.0	45.0	58.1	57.7	1451 bp	1.0	79.7
ycf1_p4	GCGCATTTGCTTGAAGGAAT	ATGGAATCGACCATTTCGCT	45.0	45.0	58.0	57.7	1094 bp	0.5	57.3
ycf1_p5	CGGGAAGTTGGAATAGGAGC	ATGGAATCGACCATTTCGCT	55.0	45.0	58.0	57.7	1436 bp	0.5	57.0