Hotspot

ndhD

SSC · 119036-120346 · 1311 bp

Back to Plectranthus

Pi 0.0175

MarkerSeek score 54.30

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1311
variable_sites: 23
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CTAATGAAGCATGGGGGTCC	ACTGCGTTGGTTGTAAGAGG	55.0	50.0	58.0	58.1	2186-2187 bp	1.0	81.2
ndhD_p2	CTAATGAAGCATGGGGGTCC	CTGCGTTGGTTGTAAGAGGT	55.0	50.0	58.0	58.1	2185-2186 bp	1.0	81.2
ndhD_p3	GAAGCATGGGGGTCCTATTG	ACTGCGTTGGTTGTAAGAGG	55.0	50.0	58.0	58.1	2181-2182 bp	1.0	81.2
ndhD_p4	GAAGCATGGGGGTCCTATTG	CTGCGTTGGTTGTAAGAGGT	55.0	50.0	58.0	58.1	2180-2181 bp	1.0	81.2
ndhD_p5	GAAGCATGGGGGTCCTATTG	GTGCCCCACCGATGTATTAG	55.0	55.0	58.0	58.1	2261-2262 bp	1.0	81.2