Hotspot

rps15-ycf1

SSC · 126832-127252 · 421 bp

Back to Plectranthus

Pi 0.0524

MarkerSeek score 70.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 421
variable_sites: 22
pi: 0.0524
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	AGTCGTTGACGTTTTCCCAA	AAAAACAGCAGCCGAGTTTC	45.0	45.0	58.0	57.5	813 bp	0.5	55.8
rps15-ycf1_p2	CCCGAGTTAGTCTTCCATGC	AAAAACAGCAGCCGAGTTTC	55.0	45.0	58.1	57.5	1004 bp	0.5	55.6
rps15-ycf1_p3	ATATTGCCCAGCTGTTCAGG	AAAAACAGCAGCCGAGTTTC	50.0	45.0	57.9	57.5	1287 bp	0.5	55.6
rps15-ycf1_p4	CCAGCTGTTCAGGTCCATTT	AAAAACAGCAGCCGAGTTTC	50.0	45.0	58.1	57.5	1280 bp	0.5	55.4
rps15-ycf1_p5	CTTCACCGTCGAGAGTAACG	AAAAACAGCAGCCGAGTTTC	55.0	45.0	58.1	57.5	1118 bp	0.5	55.1