Hotspot

infA-rps8

LSC · 84336-84460 · 125 bp

Back to Plectranthus

Pi 0.072

MarkerSeek score 71.60

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 125
variable_sites: 9
pi: 0.072
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CCATTGGGAAGCGATTCAGT	TCGGCGGAGAAATTTTATGT	50.0	40.0	58.2	55.4	213 bp	1.0	61.0
infA-rps8_p2	CCATTGGGAAGCGATTCAGT	TCGGCGGAGAAATTTTATGTT	50.0	38.1	58.2	56.2	213 bp	1.0	59.3
infA-rps8_p3	CCATTGGGAAGCGATTCAGT	ATCGGCGGAGAAATTTTATGT	50.0	38.1	58.2	56.0	214 bp	1.0	57.6
infA-rps8_p4	TAAACGAACCCGGAACATGC	TCGGCGGAGAAATTTTATGT	50.0	40.0	58.8	55.4	232 bp	1.0	56.0
infA-rps8_p5	CCATTGGGAAGCGATTCAGT	GAATCGGCGGAGAAATTTTATGT	50.0	39.1	58.2	57.8	216 bp	1.0	56.0