Hotspot

trnY-GUA-trnE-UUC

LSC · 30414-30490 · 77 bp

Back to Plectranthus

Pi 0.0519

MarkerSeek score 70.10

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 77
variable_sites: 4
pi: 0.0519
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	CGAATTTACAGTCCGTCCCC	CATAGTATGAGGGCGGTTGG	55.0	55.0	58.4	57.8	221 bp	1.0	77.3
trnY-GUA-trnE-UUC_p2	ACGAATTTACAGTCCGTCCC	CATAGTATGAGGGCGGTTGG	50.0	55.0	57.6	57.8	222 bp	1.0	77.1
trnY-GUA-trnE-UUC_p3	CGAATTTACAGTCCGTCCCC	ATAGTATGAGGGCGGTTGGT	55.0	50.0	58.4	58.2	220 bp	1.0	77.1
trnY-GUA-trnE-UUC_p4	CGAATTTACAGTCCGTCCCC	CTCTCTTTCAAGGAGGCAGC	55.0	55.0	58.4	58.3	165 bp	1.0	77.0
trnY-GUA-trnE-UUC_p5	ACGAATTTACAGTCCGTCCC	ATAGTATGAGGGCGGTTGGT	50.0	50.0	57.6	58.2	221 bp	1.0	76.9