Hotspot

atpA-atpF

LSC · 11801-11894 · 94 bp

Back to Plectranthus

Pi 0.0319

MarkerSeek score 70.90

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 94
variable_sites: 3
pi: 0.0319
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCTACTTGAAGTACGGTGCC	TGCAAGGAGCTCTAGGAACT	55.0	50.0	58.0	58.1	311-318 bp	1.0	80.3
atpA-atpF_p2	GTCGCCTACTTGAAGTACGG	TGCAAGGAGCTCTAGGAACT	55.0	50.0	58.1	58.1	315-322 bp	1.0	79.6
atpA-atpF_p3	CCGTCGCCTACTTGAAGTAC	TGCAAGGAGCTCTAGGAACT	55.0	50.0	58.1	58.1	317-324 bp	1.0	79.5
atpA-atpF_p4	CCTACTTGAAGTACGGTGCC	TTGGGGGCGATGAAAGAAAT	55.0	45.0	58.0	57.8	231-238 bp	1.0	79.1
atpA-atpF_p5	CGTCGGCTCGAATAGTTACC	TGCAAGGAGCTCTAGGAACT	55.0	50.0	58.2	58.1	226-233 bp	1.0	78.6