Hotspot

rps16-trnQ-UUG

LSC · 5884-6937 · 1054 bp

Back to Plectranthus

Pi 0.0389

MarkerSeek score 73.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1054
variable_sites: 36
pi: 0.0389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1006-1127 bp	1.0	85.0
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1010-1131 bp	1.0	80.2
rps16-trnQ-UUG_p3	TATCCTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.3	58.1	1014-1135 bp	1.0	80.2
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1036-1157 bp	1.0	80.1
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	998-1119 bp	1.0	79.8