primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.171606	84	350	717-749	1.000000	82.1
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	20	20	55.0	50.0	58.1	58.0	0.0	10.2	0.177364	84	350	716-748	1.000000	82.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.175967	84	350	731-763	1.000000	82.0
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	84	350	730-762	1.000000	82.0
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	20	20	50.0	50.0	57.9	58.0	0.0	10.2	0.177475	84	350	732-764	1.000000	82.0
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5884	6937	1006-1127	1.000000	85.0
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	20	20	45.0	50.0	58.1	57.3	8.1	16.9	0.806797	5884	6937	1010-1131	1.000000	80.2
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TATCCTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.3	58.1	0.0	16.9	0.812245	5884	6937	1014-1135	1.000000	80.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	20	20	45.0	50.0	58.1	58.7	8.1	0.0	0.818817	5884	6937	1036-1157	1.000000	80.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.863824	5884	6937	998-1119	1.000000	79.8
atpA-atpF_p1	atpA-atpF	1	CCTACTTGAAGTACGGTGCC	TGCAAGGAGCTCTAGGAACT	20	20	55.0	50.0	58.0	58.1	0.0	14.6	0.061793	11801	11894	311-318	1.000000	80.3
atpA-atpF_p2	atpA-atpF	2	GTCGCCTACTTGAAGTACGG	TGCAAGGAGCTCTAGGAACT	20	20	55.0	50.0	58.1	58.1	13.2	14.6	0.150373	11801	11894	315-322	1.000000	79.6
atpA-atpF_p3	atpA-atpF	3	CCGTCGCCTACTTGAAGTAC	TGCAAGGAGCTCTAGGAACT	20	20	55.0	50.0	58.1	58.1	0.0	14.6	0.150373	11801	11894	317-324	1.000000	79.5
atpA-atpF_p4	atpA-atpF	4	CCTACTTGAAGTACGGTGCC	TTGGGGGCGATGAAAGAAAT	20	20	55.0	45.0	58.0	57.8	0.0	0.0	0.236100	11801	11894	231-238	1.000000	79.1
atpA-atpF_p5	atpA-atpF	5	CGTCGGCTCGAATAGTTACC	TGCAAGGAGCTCTAGGAACT	20	20	55.0	50.0	58.2	58.1	0.0	14.6	0.304271	11801	11894	226-233	1.000000	78.6
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CGAATTTACAGTCCGTCCCC	CATAGTATGAGGGCGGTTGG	20	20	55.0	55.0	58.4	57.8	0.0	3.9	0.521328	30414	30490	221-221	1.000000	77.3
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	ACGAATTTACAGTCCGTCCC	CATAGTATGAGGGCGGTTGG	20	20	50.0	55.0	57.6	57.8	0.0	3.9	0.545005	30414	30490	222-222	1.000000	77.1
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CGAATTTACAGTCCGTCCCC	ATAGTATGAGGGCGGTTGGT	20	20	55.0	50.0	58.4	58.2	0.0	0.0	0.548943	30414	30490	220-220	1.000000	77.1
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	CGAATTTACAGTCCGTCCCC	CTCTCTTTCAAGGAGGCAGC	20	20	55.0	55.0	58.4	58.3	0.0	0.0	0.614884	30414	30490	165-165	1.000000	77.0
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	ACGAATTTACAGTCCGTCCC	ATAGTATGAGGGCGGTTGGT	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.572620	30414	30490	221-221	1.000000	76.9
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TGTGATACCAAGGAGAACTGT	ATGCTAGTTCTTGCTCCAGC	21	20	42.9	50.0	56.3	58.0	0.0	7.6	2.744341	37179	37978	988-988	0.500000	38.0
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	TGTGATACCAAGGAGAACTGT	TAGTACGTCGTCCTTCCCTG	21	20	42.9	55.0	56.3	58.3	0.0	0.0	2.966050	37179	37978	1021-1021	0.500000	36.3
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TGTGATACCAAGGAGAACTGT	GTCGTCCTTCCCTGAAATGG	21	20	42.9	55.0	56.3	58.3	0.0	0.0	2.969744	37179	37978	1015-1015	0.500000	36.2
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	ACTGTAGAAAGTAAAGGGTGCT	ATGCTAGTTCTTGCTCCAGC	22	20	40.9	50.0	57.0	58.0	0.0	7.6	3.012070	37179	37978	972-972	0.500000	35.9
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TGTGATACCAAGGAGAACTGT	TCGTCCTTCCCTGAAATGGA	21	20	42.9	50.0	56.3	58.3	0.0	0.0	3.057074	37179	37978	1014-1014	0.500000	35.5
psaA-pafI_p1	psaA-pafI	1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	57.9	57.9	4.1	0.0	0.244113	46126	46955	911-942	1.000000	83.7
psaA-pafI_p2	psaA-pafI	2	GAAAAATGACCCGGTTTGGC	ATTGGTTGAAGATCACGGGG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.338015	46126	46955	929-960	1.000000	82.8
psaA-pafI_p3	psaA-pafI	3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	57.9	57.7	4.1	19.9	0.390477	46126	46955	984-1015	1.000000	82.1
psaA-pafI_p4	psaA-pafI	4	GAAAAATGACCCGGTTTGGC	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	58.2	57.7	0.0	19.9	0.484379	46126	46955	1002-1033	1.000000	81.2
psaA-pafI_p5	psaA-pafI	5	GCCCATTCCTCGAAAGAAGT	CGGAGGCTTGGTTTGATCAA	20	20	50.0	50.0	57.9	58.5	4.1	3.4	0.581718	46126	46955	992-1023	1.000000	80.5
trnW-CCA-trnP-UGG_p1	trnW-CCA-trnP-UGG	1	GTTTTGGAGACCCACGTTCT	GGGTACAAAATGTCACGGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.136582	71145	71311	247-248	1.000000	81.2
trnW-CCA-trnP-UGG_p2	trnW-CCA-trnP-UGG	2	GTTTTGGAGACCCACGTTCT	TGGGTACAAAATGTCACGGG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.137926	71145	71311	248-249	1.000000	81.2
trnW-CCA-trnP-UGG_p3	trnW-CCA-trnP-UGG	3	GTTTTGGAGACCCACGTTCT	GTCACGGGTTCAAATCCTGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.144618	71145	71311	236-237	1.000000	81.2
trnW-CCA-trnP-UGG_p4	trnW-CCA-trnP-UGG	4	GTTTTGGAGACCCACGTTCT	CACGGGTTCAAATCCTGTCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	71145	71311	234-235	1.000000	81.2
trnW-CCA-trnP-UGG_p5	trnW-CCA-trnP-UGG	5	GTTTTGGAGACCCACGTTCT	TGTCACGGGTTCAAATCCTG	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.145952	71145	71311	237-238	1.000000	81.2
psbT-pbf1_p1	psbT-pbf1	1	CGAGAACCACCTAAAGTTCCA	GGGTACGCCTTATATACCGC	21	20	47.6	55.0	57.6	57.7	0.0	0.0	1.680523	78691	78755	173-173	1.000000	69.3
psbT-pbf1_p2	psbT-pbf1	2	CGAGAACCACCTAAAGTTCCA	GATCCATTCGAGGAACACGG	21	20	47.6	55.0	57.6	58.4	0.0	0.0	1.824342	78691	78755	125-125	1.000000	69.2
psbT-pbf1_p3	psbT-pbf1	3	CGAGAACCACCTAAAGTTCCA	GTTTTACCGGGTACGCCTTA	21	20	47.6	50.0	57.6	57.6	0.0	6.3	1.774705	78691	78755	181-181	1.000000	68.5
psbT-pbf1_p4	psbT-pbf1	4	CGAGAACCACCTAAAGTTCCA	TAAGTTTTACCGGGTACGCC	21	20	47.6	50.0	57.6	57.6	0.0	6.3	1.774705	78691	78755	184-184	1.000000	68.4
psbT-pbf1_p5	psbT-pbf1	5	CGAGAACCACCTAAAGTTCCA	CGGGTACGCCTTATATACCG	21	20	47.6	55.0	57.6	57.5	0.0	4.1	1.945894	78691	78755	174-174	1.000000	67.2
rpl36-infA_p1	rpl36-infA	1	AATTACTCTAATGCGCCCCC	TCGACTCCGAAACAAGGATT	20	20	50.0	45.0	57.7	56.9	4.2	0.0	1.393918	84007	84101	204-204	1.000000	70.8
rpl36-infA_p2	rpl36-infA	2	AATTACTCTAATGCGCCCCC	TCGACTCCGAAACAAGGATTT	20	21	50.0	42.9	57.7	57.6	4.2	0.0	1.668204	84007	84101	204-204	1.000000	68.6
rpl36-infA_p3	rpl36-infA	3	AATTACTCTAATGCGCCCCC	CGACTCCGAAACAAGGATTTG	20	21	50.0	47.6	57.7	57.5	4.2	0.0	1.727563	84007	84101	203-203	1.000000	68.1
rpl36-infA_p4	rpl36-infA	4	AATTACTCTAATGCGCCCCC	ATGATTCAACCAGAGGACGT	20	20	50.0	45.0	57.7	56.5	4.2	0.0	1.724798	84007	84101	232-232	1.000000	67.9
rpl36-infA_p5	rpl36-infA	5	AATTACTCTAATGCGCCCCC	CGACTCCGAAACAAGGATTT	20	20	50.0	45.0	57.7	56.1	4.2	0.0	2.194063	84007	84101	203-203	1.000000	64.4
infA-rps8_p1	infA-rps8	1	CCATTGGGAAGCGATTCAGT	TCGGCGGAGAAATTTTATGT	20	20	50.0	40.0	58.2	55.4	16.2	7.2	2.796913	84336	84460	213-213	1.000000	61.0
infA-rps8_p2	infA-rps8	2	CCATTGGGAAGCGATTCAGT	TCGGCGGAGAAATTTTATGTT	20	21	50.0	38.1	58.2	56.2	16.2	7.2	3.004917	84336	84460	213-213	1.000000	59.3
infA-rps8_p3	infA-rps8	3	CCATTGGGAAGCGATTCAGT	ATCGGCGGAGAAATTTTATGT	20	21	50.0	38.1	58.2	56.0	16.2	7.2	3.225251	84336	84460	214-214	1.000000	57.6
infA-rps8_p4	infA-rps8	4	TAAACGAACCCGGAACATGC	TCGGCGGAGAAATTTTATGT	20	20	50.0	40.0	58.8	55.4	0.7	7.2	3.393659	84336	84460	232-232	1.000000	56.0
infA-rps8_p5	infA-rps8	5	CCATTGGGAAGCGATTCAGT	GAATCGGCGGAGAAATTTTATGT	20	23	50.0	39.1	58.2	57.8	16.2	7.2	3.414657	84336	84460	216-216	1.000000	56.0
rpl16_p1	rpl16	1	CATATCGGCGATTGCTAGCT	CGACGGAAAAGAAATTGCCC	20	20	50.0	50.0	58.0	58.0	28.9	0.0	0.024624	85526	86802	1802-1810	1.000000	82.3
rpl16_p2	rpl16	2	CATATCGGCGATTGCTAGCT	GACGGAAAAGAAATTGCCCG	20	20	50.0	50.0	58.0	58.0	28.9	0.0	0.024624	85526	86802	1801-1809	1.000000	82.3
rpl16_p3	rpl16	3	AGCATATCGGCGATTGCTAG	CGACGGAAAAGAAATTGCCC	20	20	50.0	50.0	58.0	58.0	35.6	0.0	0.024624	85526	86802	1804-1804	0.500000	59.8
rpl16_p4	rpl16	4	AGCATATCGGCGATTGCTAG	GACGGAAAAGAAATTGCCCG	20	20	50.0	50.0	58.0	58.0	35.6	0.0	0.024624	85526	86802	1803-1803	0.500000	59.8
rpl16_p5	rpl16	5	GAGCATATCGGCGATTGCTA	GACGGAAAAGAAATTGCCCG	20	20	50.0	50.0	58.0	58.0	36.0	0.0	0.026520	85526	86802	1804-1804	0.500000	59.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	TCTCTTTCTACCGGGATTTCA	TCCTAAGAGCAGCGTGTCTA	21	20	42.9	50.0	56.1	57.9	6.3	0.0	3.037505	116912	117670	855-858	1.000000	60.5
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	TCTCTTTCTACCGGGATTTCA	AGCAGCGTGTCTACCAATTT	21	20	42.9	45.0	56.1	57.8	6.3	0.0	3.098232	116912	117670	848-851	1.000000	60.0
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	TCTCTTTCTACCGGGATTTCA	CCTAAGAGCAGCGTGTCTAC	21	20	42.9	55.0	56.1	57.8	6.3	0.0	3.109523	116912	117670	854-857	1.000000	59.9
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	TCTCTTTCTACCGGGATTTCA	CTAAGAGCAGCGTGTCTACC	21	20	42.9	55.0	56.1	57.8	6.3	0.0	3.109523	116912	117670	853-856	1.000000	59.9
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	TCTCTTTCTACCGGGATTTCA	GAGCAGCGTGTCTACCAATT	21	20	42.9	50.0	56.1	58.3	6.3	0.0	3.186375	116912	117670	849-852	1.000000	59.3
ccsA-ndhD_p1	ccsA-ndhD	1	CTAATGAAGCATGGGGGTCC	TCAGTAGCTTTTCGATGGCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.037293	118807	119035	830-831	1.000000	83.5
ccsA-ndhD_p2	ccsA-ndhD	2	GAAGCATGGGGGTCCTATTG	TCAGTAGCTTTTCGATGGCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.037293	118807	119035	825-826	1.000000	83.5
ccsA-ndhD_p3	ccsA-ndhD	3	CTAATGAAGCATGGGGGTCC	CAGTAGCTTTTCGATGGCCT	20	20	55.0	50.0	58.0	58.0	0.0	3.3	0.042956	118807	119035	829-830	1.000000	83.4
ccsA-ndhD_p4	ccsA-ndhD	4	GAAGCATGGGGGTCCTATTG	CAGTAGCTTTTCGATGGCCT	20	20	55.0	50.0	58.0	58.0	0.0	3.3	0.042956	118807	119035	824-825	1.000000	83.4
ccsA-ndhD_p5	ccsA-ndhD	5	GAAGCATGGGGGTCCTATTG	GGTTGATAATAGTGGGCGCA	20	20	55.0	50.0	58.0	58.0	0.0	10.8	0.051324	118807	119035	1122-1123	1.000000	82.9
ndhD_p1	ndhD	1	CTAATGAAGCATGGGGGTCC	ACTGCGTTGGTTGTAAGAGG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.134165	119036	120346	2186-2187	1.000000	81.2
ndhD_p2	ndhD	2	CTAATGAAGCATGGGGGTCC	CTGCGTTGGTTGTAAGAGGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.134165	119036	120346	2185-2186	1.000000	81.2
ndhD_p3	ndhD	3	GAAGCATGGGGGTCCTATTG	ACTGCGTTGGTTGTAAGAGG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.134165	119036	120346	2181-2182	1.000000	81.2
ndhD_p4	ndhD	4	GAAGCATGGGGGTCCTATTG	CTGCGTTGGTTGTAAGAGGT	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.134165	119036	120346	2180-2181	1.000000	81.2
ndhD_p5	ndhD	5	GAAGCATGGGGGTCCTATTG	GTGCCCCACCGATGTATTAG	20	20	55.0	55.0	58.0	58.1	0.0	0.0	0.134165	119036	120346	2261-2262	1.000000	81.2
ndhG-ndhI_p1	ndhG-ndhI	1	AATCCCAATGAAAAGGCCGA	CATGATTACCCTGTCCCACG	20	20	45.0	55.0	57.8	58.0	0.0	0.0	0.276571	122196	122563	914-920	1.000000	80.5
ndhG-ndhI_p2	ndhG-ndhI	2	ATCCCAATGAAAAGGCCGAA	CATGATTACCCTGTCCCACG	20	20	45.0	55.0	57.8	58.0	0.0	0.0	0.276571	122196	122563	913-919	1.000000	80.5
ndhG-ndhI_p3	ndhG-ndhI	3	TCCCAATGAAAAGGCCGAAT	CATGATTACCCTGTCCCACG	20	20	45.0	55.0	57.8	58.0	0.0	0.0	0.276571	122196	122563	912-918	1.000000	80.5
ndhG-ndhI_p4	ndhG-ndhI	4	ATCCCAATGAAAAGGCCGAA	ACATCAGAACGTTTCCGAGG	20	20	45.0	50.0	57.8	57.9	0.0	0.5	0.310416	122196	122563	846-852	1.000000	80.4
ndhG-ndhI_p5	ndhG-ndhI	5	TCCCAATGAAAAGGCCGAAT	ACATCAGAACGTTTCCGAGG	20	20	45.0	50.0	57.8	57.9	0.0	0.5	0.310416	122196	122563	845-851	1.000000	80.4
rps15-ycf1_p1	rps15-ycf1	1	AGTCGTTGACGTTTTCCCAA	AAAAACAGCAGCCGAGTTTC	20	20	45.0	45.0	58.0	57.5	3.2	8.2	0.519023	126832	127252	813-813	0.500000	55.8
rps15-ycf1_p2	rps15-ycf1	2	CCCGAGTTAGTCTTCCATGC	AAAAACAGCAGCCGAGTTTC	20	20	55.0	45.0	58.1	57.5	0.0	8.2	0.550694	126832	127252	1004-1004	0.500000	55.6
rps15-ycf1_p3	rps15-ycf1	3	ATATTGCCCAGCTGTTCAGG	AAAAACAGCAGCCGAGTTTC	20	20	50.0	45.0	57.9	57.5	13.8	8.2	0.554990	126832	127252	1287-1287	0.500000	55.6
rps15-ycf1_p4	rps15-ycf1	4	CCAGCTGTTCAGGTCCATTT	AAAAACAGCAGCCGAGTTTC	20	20	50.0	45.0	58.1	57.5	13.8	8.2	0.576878	126832	127252	1280-1280	0.500000	55.4
rps15-ycf1_p5	rps15-ycf1	5	CTTCACCGTCGAGAGTAACG	AAAAACAGCAGCCGAGTTTC	20	20	55.0	45.0	58.1	57.5	0.0	8.2	0.612373	126832	127252	1118-1118	0.500000	55.1
ycf1_p1	ycf1	1	GCCGAAAGTCTGATTGTTGC	ATGGAATCGACCATTTCGCT	20	20	50.0	45.0	58.0	57.7	0.0	1.8	0.362960	130959	131078	1019-1019	1.000000	80.6
ycf1_p2	ycf1	2	AACCTTTGATTAGACCCCGC	ATGGAATCGACCATTTCGCT	20	20	50.0	45.0	57.9	57.7	0.0	1.8	0.456340	130959	131078	1037-1037	1.000000	79.9
ycf1_p3	ycf1	3	AATCCTCAGTCCATTCGGGA	ATGGAATCGACCATTTCGCT	20	20	50.0	45.0	58.1	57.7	0.0	1.8	0.452488	130959	131078	1451-1451	1.000000	79.7
ycf1_p4	ycf1	4	GCGCATTTGCTTGAAGGAAT	ATGGAATCGACCATTTCGCT	20	20	45.0	45.0	58.0	57.7	0.3	1.8	0.332492	130959	131078	1094-1094	0.500000	57.3
ycf1_p5	ycf1	5	CGGGAAGTTGGAATAGGAGC	ATGGAATCGACCATTTCGCT	20	20	55.0	45.0	58.0	57.7	0.0	1.8	0.377136	130959	131078	1436-1436	0.500000	57.0