Hotspot

ycf1

SSC · 126007-131274 · 5268 bp

Back to Platymiscium

Pi 0.0385

MarkerSeek score 61.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5268
variable_sites: 202
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AACTTTGGCCCTGTTTAGAA	TATGTTTTACGTATCCACCC	40.0	40.0	55.0	52.4	185 bp	1.0	46.1
ycf1_p2	ACTTTGGCCCTGTTTAGAAA	TATGTTTTACGTATCCACCC	40.0	40.0	55.0	52.4	184 bp	1.0	46.1
ycf1_p3	ACTTTGGCCCTGTTTAGAAA	AGAATATGTTTTACGTATCCACCC	40.0	37.5	55.0	56.6	188 bp	1.0	46.0
ycf1_p4	AACTTTGGCCCTGTTTAGAA	AGAATATGTTTTACGTATCCACCC	40.0	37.5	55.0	56.6	189 bp	1.0	45.9
ycf1_p5	AACTTTGGCCCTGTTTAGAAA	AGAATATGTTTTACGTATCCACCC	38.1	37.5	55.9	56.6	189 bp	1.0	45.9