Hotspot

ndhF-rpl32

SSC · 114517-115110 · 594 bp

Back to Platymiscium

Pi 0.0596

MarkerSeek score 77.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 594
variable_sites: 33
pi: 0.0596
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACTCGGAAAAGCCCACATAC	TACGCTTTCAATGCTGTCCA	50.0	45.0	57.9	57.8	819-821 bp	1.0	85.5
ndhF-rpl32_p2	ACTCGGAAAAGCCCACATAC	TGCTGTCCAATATCCCCTCT	50.0	50.0	57.9	57.8	808-810 bp	1.0	85.5
ndhF-rpl32_p3	GGACGTGTGAACCAAAAACC	TACGCTTTCAATGCTGTCCA	50.0	45.0	57.8	57.8	1199-1201 bp	1.0	83.1
ndhF-rpl32_p4	GCAATAGGACGTGTGAACCA	TACGCTTTCAATGCTGTCCA	50.0	45.0	58.2	57.8	1205-1207 bp	1.0	83.0
ndhF-rpl32_p5	TGCAATAGGACGTGTGAACC	TACGCTTTCAATGCTGTCCA	50.0	45.0	58.2	57.8	1206-1208 bp	1.0	83.0